Table 7. Primers used in mutational analysis of TLR4 and TLR5.

Primer	Manufacturer		Direction	Primer sequence
Full-length TLR2	MWG Biotech (London, UK)		Forward	5′-GGA CAA TGT CAC GTG TTT TG-3′
			Reverse	5′-CGA ATC TAG GAT TTT ATT GCT GT-3′
Full-length TLR5	MWG Biotech (London, UK)		Forward	5′-TTT CCG TCC CGC AGG ATC ATG-3′
			Reverse	5′-ACC CGT GCA AAT GGC AAG AG-3′
Full-length TLR4	MWG Biotech (London, UK)	TLR4 Exon 1	Forward	5′-TTC CTC TTG CCC CTT AA CTC-3′
			Reverse	5′-GCC ATG TAA CCA TGA ACT GT-3′
		TLR4 Exon 2	Forward	5′-GAT GGA TGG ATG GAC AGA CC-3′
			Reverse	5′-TTC ACA GAT GAG GCA ATG GG-3′
		TLR4 Exon 3	Forward	5′-ATA CTG AAT CTG TGG GGC TT-3′
			Reverse	5′-ATT TGG TGG AAG CAT CCA CCT-3′

Primers used in the TLR2/TLR4 and TLR5 mutational analysis.