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. 2010 Dec 23;5(12):e15692. doi: 10.1371/journal.pone.0015692

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Fransen et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Mice were immunized with whole cell pertussis vaccine (Bordetella pertussis) and antigen-specific titers of IgG, IgG1, IgG2a/c, IgG2b, and IgG3 in sera were determined with ELISA. Also total IgE in sera of mice immunized with PBS or whole cell pertussis vaccine was measured with ELISA. Results for C57BL/6, TLR2−/−, and TRIF-deficient (TRIFdef) mice are shown in panel A, results for C3H/HeOuJ and C3H/HeJ mice are shown in panel B. Data are expressed as means of log₁₀ titers for 6 mice per group. An asterisk indicates a significant difference compared to the wild type group, * indicates p<0.05, ** indicates p<0.01.