Figure 2. Centrosome irradiation causes a distinct change in cell morphology over a 2 hour period.

A,E show GFP fluorescence images of cell prior to irradiation. B,F show fluorescence images of cell immediately following irradiation. The inset represents a magnified image of the centrosome region exposed to the laser. Loss of fluorescence at these positions following laser exposure is evident in magnified insets. C,G show phase contrast images of cell immediately following laser irradiation. Cells do not appear to be affected by laser exposure. D,H show the phase contrast images of cells observed for 2 or more hours following centrosome irradiation. In 22 of 26 centrosome irradiated cells, morphology appears less polarized when compared to images taken immediately following irradiation. Scale bar  = 10 µm.