Fig. 1.

Deletion of V_H to D_H intergenic region leads to generation of long V_H- and D-containing transcripts. (A) Schematic diagram of proximal WT murine 129sVe IgH locus before (Upper) and after gene targeting to delete the 109-kb intergenic region (Lower). The order of the D segments is indicated. (B) Northern analyses of total RNA from WT (^+/+), ΔV_H-D/+, and ΔV_H-D/ΔV_H-D thymocytes (Left) as well as total RNA from RAG2^−/−(^+/+) and RAG2^−/−(ΔV_H-D/ΔV_H-D) A-MuLV-transformed pro-B lines after probing with (from top to bottom): a 1-kb fragment encompassing DSP2.9 (see A); a 511-bp fragment derived from the germ-line V_H7183.a4.6 segment (see A); a 631-bp probe derived from the germ line corresponding to the V_HJ558.29 segment; and a GAPDH cDNA. Ribosomal RNA loading is shown at the bottom as additional loading control.