Fig. 4.
VH to DH intergenic deletion up-regulates DH to JH recombination in thymocytes. (A) Two sets of independent splenic T-cell hybridomas were obtained from WT (n = 186), ΔVH-D/+ (n = 188), and ΔVH-D/ΔVH-D (n = 167) mice. DNA was digested with StuI, and Southern blot analysis was performed with an EcoRI/HindIII fragment probe downstream of JH4. The germ-line JH locus yields a 6.2-kb band (GL), and DH to JH rearrangements are scored as rearranged bands with a size distinct from the germ-line band. DNA from the fusion partner BW5147 and kidney DNA is shown as controls. A representative set of analyses is shown. (B) Percentage of cells containing monoallelic (red) and biallelic (blue) JH rearrangements as determined by either one or two non-germ-line bands in analyses of A. The average of two independent experiments is shown.