Fig. 5.
Deletion of the VH to DH intergenic region leads to VH to DJH recombination in thymocytes. (A) PCR assay for VH to DJH for indicated gene families (Right) in DNA from sorted DP-T cells. Size of rearrangements to various J segments is indicated on Left. Analysis of Igκ rearrangement (VJκ) controlled for potential B cell contamination. (B) The level of VH to DJH rearrangements in the same panel of hybridomas shown in Fig. 4 was determined by a combination of hybridization to a JH probe, a probe between VH and DH segments, plus PCR for proximal VH to DJH rearrangements in each hybridoma (see SI Materials and Methods for details).