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. 2010 Dec 2;107(51):22243–22248. doi: 10.1073/pnas.1009479107

Fig. 1.

Fig. 1.

AEG-1 induces autophagy in IM-PHFA cells. (A) IM-PHFA cells were transfected with GFP-LC3 and infected with different pfu/cell of Ad.AEG-1 for 48 h, localization of LC3 in transfected cells was examined by confocal microscopy (magnification 100×), and autophagosome formation was quantified and data presented as percentage of GFP-LC3–transfected cells with punctate fluorescence to autophagosome formation. A minimum of 100 GFP-LC3–transfected cells were counted. *P < 0.05, compared with control. (B) The MDC fluorescent intensity of Ad.AEG-1–treated IM-PHFA cells was analyzed by flow cytometry. This result was representative of three different experiments. (C) IM-PHFA cells were infected with Ad.AEG-1 or Ad.vec for 48 h, fixed, and processed for electron microscopy (single arrow, autophagosome; control and others, 2 μm). (D) The numbers of autophagosomes in IM-PHFA cells 48 h after Ad.AEG-1 infection, the values are the means ± SD of three independent experiments. Asterisk indicates statistically significant change vs. corresponding control.