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. 2010 Dec 2;107(51):22243–22248. doi: 10.1073/pnas.1009479107

Fig. 3.

Fig. 3.

AEG-1 induces noncanonical autophagy in IM-PHFA cells. (A) IM-PHFA cells were transfected with the indicated siRNAs and LC3-GFP followed by infection with 50 pfu/cell Ad.AEG-1 or Ad.vec and cytoplasmic aggregation of LC3-GFP was determined. A minimum of 100 GFP-LC3–transfected cells were counted. *P < 0.05; **P < 0.001, compared with sicontrol. (B) LC3-II expression was determined 48 h after administration of the indicated siRNAs and Ad.AEG-1 infection (50 pfu/cell) by immunoblotting. IM-PHFA Cells were infected with Ad.AEG-1 or Ad.vec for 48 h and mRNA (C) and protein (D) expression of Beclin-1 and ATG5 were analyzed using Taqman real-time PCR and Western blotting, respectively. Values are the mean ± SD of three independent experiments and *P < 0.05; **P < 0.001 vs. control cells.