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Inhibition of autophagy resulted in serum starvation-induced cell death. IM-PHFA cells were transfected with siATG5 and MTT assay for cell viability (A), Annexin V assay for apoptosis (B), and Caspase-Glo(R) 3/7 assay for caspase-3 expression (C) was performed 48 h after infection with Ad.AEG-1 (10, 20, or 50 pfu/cell) or Ad.vec (50 pfu/cell), followed by 4 d after serum starvation (0.1%). *P < 0.05, compared with sicontrol.
