Fig. 1.

SSRP1 and Spt16 play a critical role in CSR. (A) Flow cytometry (FACS) profile of the IgA switching population following the introduction of the indicated RNAi oligonucleotide introduction under CIT(−) and (+) conditions. (B) Summarized data of IgA switching population of various gene knockdowns with respect to AID in CIT(−) and (+) conditions. Numbers 1 and 2 represent two independent RNAi oligonucleotides. SD values were determined from three independent experiments. (C) Knockdown efficiency of the indicated gene was quantified by immunobloting. (D) μGLT, αGLT, and AID transcripts were quantified by real-time PCR normalized to hypoxanthine-guanine phosphoribosyltransferase (HPRT) following the introduction of the indicated RNAi oligonucleotide. SD values were derived from three independent experiments.