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. 2010 Dec 7;107(51):22190–22195. doi: 10.1073/pnas.1016923108

Fig. 5.

Fig. 5.

H3K4me3 is essential for S region DNA cleavage. (A) DNA break assay by γH2AX ChIP. The ChIP assay was performed in SSRP1 knockdown and control samples using an anti-γH2AX antibody. The pulled-down DNA was subjected to S- and Sα-specific detection by real-time PCR normalized to the fraction of γH2AX in H2AX, followed by the maximal value in each dataset. (B) The biotin-labeling break assay was performed in SSRP1 knockdown, Spt16 knockdown, and control samples. The pulled-down DNA was subjected to Sμ- and Sα specific detection by real-time PCR normalized to the input DNA. SD values were derived from three independent experiments. (C and D) The ChIP assay was performed in Wdr5 or Wdr5 and ASH2 knockdown and control samples using the anti-γH2AX antibody. (E) Biotin-labeling break assay was performed in Wdr5 and ASH2 knockdown and control samples.