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. 2010 Dec 6;107(51):22350–22355. doi: 10.1073/pnas.1012795108

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Fig. 2. — fiona mutant female gametophytes give rise to viable seeds. (A) Analysis of siliques resulting from crosses of fiona/FIONA and FIONA/FIONA plants. (B) Segregation analysis of fiona, as determined by reciprocal crosses between fiona/FIONA and FIONA/FIONA plants and PCR-based genotyping of the progeny. TE, transmission efficiency. (C) Flow cytometry ploidy analysis of seeds derived from wild-type plants. (D) Flow cytometry ploidy analysis of seeds derived from fiona/FIONA plants. (E) Flow cytometry ploidy analysis of seeds derived from emasculated fie/FIE flowers. (F–I) Fertilization of a fiona mutant gametophyte expressing pAt5g40260::NLS_GFP with sperm cells expressing HTR10-mRFP1. Arrows point at the polar nuclei. (F) Bright-field image. (G) pAT5g40260::NLS_GFP. (H) HTR10-mRFP1. (I) Overlay.