Fig. 1.
Induced deletion of DDB1 deprived hepatocytes of replicative capacity during liver development and regeneration. (A) PCR analysis of floxed (DDB1F) and deleted DDB1 (DDB1Δ) alleles in liver genomic DNA prepared from adult mice with indicated genotypes 1 or 2 d after poly(I:C) injection. Tail DNA from heterozygous DDB1F/Δ mice was used as a quantitative control for the two alleles. (B) Western analysis of DDB1 protein levels in whole liver lysates from control (c, DDB1F/F) or mutant (m, DDB1F/F;Mx1-Cre+/−) mice 1, 2, 3, or 5 d after poly(I:C) injection. (C) IHC staining for incorporated BrdU on liver sections from 1-mo-old mice 3 d after injection. Arrows indicate positive hepatocytes. (D) BrdU IHC staining on liver sections from 2-mo-old mice 20, 40, or 70 h after PH surgery. Surgery was performed on mice 3 d after injection with poly(I:C).