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. 2010 Nov 13;134(1):196–209. doi: 10.1093/brain/awq314

Figure 4.

Figure 4

Generation of mice expressing wild-type human SOD1 targeted to the mitochondrial intermembrane space. (A) Schematic representation of the strategy to create the mitoSOD1 transgene cloned in the mouse prion promoter vector. (B) Detection of mitoSOD1 in non-transgenic (−) and transgenic (+) mouse tissues by western blot, using a sheep polyclonal SOD1 antibody that recognizes both transgenic human mitoSOD1 and endogenous mouse SOD1 (mSOD1). WT hSOD1 = wild-type human SOD1; pre = mitochondrial targeting presequence; TM = transmembrane domain; L = 2 amino acid linker; MMP = matrix metalloproteinases.