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. 2010 Dec 22;134(1):258–277. doi: 10.1093/brain/awq341

Figure 1.

Figure 1

Abnormal lysosome-related compartments in the brains of TgCRND8. Sections from the hippocampal CA1 sector of 2- (A1), 6- (A2) and 12-month-old (A3) TgCRND8 (strain 129S6) and 12-month-old wild-type (WT) (A4) mice (n = 3–6 mice per age per genotype) immunostained with an antibody against mouse cathepsin D (RU2). Representative microphotograph images depict abundant abnormally enlarged lysosome-related granules in CA1 neurons of TgCRND8 at 6- and 12-months of age. (B) Images of the CA1 sector from toluidine blue-stained 1 µm thick sections that were derived from vibratome sections of 12-month-old TgCRND8 (B1) and wild-type (B2) immunostained with RU2. Toluidine blue-stained structures are in blue/purple and RU2-stained granules are in black and denoted by arrowheads. (C) Vibratome brain sections from 6-month-old TgCRND8 were immunostained with the anti-cathepsin D antibody RU2 and then processed for electron microscopy embedding. Areas of the hippocampal CA1 sector containing RU2-positive giant granules (as those seen in Fig. 1A2) were selected for ultrathin sectioning. The electron micrograph shows that a neuronal soma (asterisk) contains two giant RU2-positive granules decorated by strong diaminobenzidine products (arrows). (D) Electron micrographs of the CA1 hippocampal area in 6-month-old TgCRND8 mice depict giant granules (arrows) with single-limiting membranes, containing undigested materials. Scale bars: 10 µm (A and B); 2 µm (C, D1 and D3); 500 nm (D2). CatD = cathepsin D; CRND8 = TgCRND8.