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. 2010 Dec 22;134(1):258–277. doi: 10.1093/brain/awq341

Figure 5.

Figure 5

Cystatin B deletion elevating lysosomal protein degradation. (A) Rates of proteolysis of long-lived proteins in fibroblasts from wild-type (WT) and CBKO (CB−/−) mice maintained in the presence (A1) or absence (A2) of serum. Where indicated a combination of ammonium chloride and leupeptin (leup) was added to inhibit proteolysis in the lysosomal compartment. Values are mean + SEM of three different experiments with triplicate samples. Mean differences between the untreated wild-type and CBKO (i.e. without lysosomal inhibitors) at each time point were analysed by two-tailed Student’s t-test. *P < 0.05. (B) Rates of proteolysis of long-lived proteins in primary neurons from wild-type and CBKO mice maintained in neurobasal medium supplemented with B27. Mean differences between wild-type and CBKO at each time point were analysed by two-tailed Student’s t-test. *P < 0.05.