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. 2010 Dec 22;134(1):258–277. doi: 10.1093/brain/awq341

Figure 9.

Figure 9

Reduction of amyloid load and Aβ levels in the brains of CBKO/TgCRND8. Immunocytochemistry using antibodies to Aβ40 (A) or Aβ42 (B) revealed reduction of amyloid deposition in 6-month-old CBKO/TgCRND8 compared to age-matched TgCRND8. (A3 and B3) Results of quantification of amyloid load detected with antibodies to Aβ40 (A3) or Aβ42 (B3) in the cerebral cortex (CTX) and the hippocampus (Hipp) from TgCRND8 (n = 10; 9 males + 1 female) and CBKO/TgCRND8 (n = 12; 3 males + 9 females), expressed as the percent difference from TgCRND8. Values are the mean ± SEM for each group. Significant differences were analysed by two-tailed Student’s t-test. *P < 0.005, **P < 0.001. (C) Levels of Aβ40 and Aβ42 in the brains of 6-month-old TgCRND8 (n = 11; 9 males + 2 females) and CBKO/TgCRND8 (n = 13; 3 males + 10 females) were measured in formic acid-extracted, sucrose homogenates using an enzyme-linked immunosorbent assay method. Values are the mean ± SEM for each group. Significant differences were analysed by two-tailed Student’s t-test. (D) Western blotting (WB) using the antibody JRF/Aβtot/17 (top) on brain homogenates from 6-month-old TgCRND8 and CBKO/TgCRND8. The glyceraldehyde 3-phosphate dehydrogenase (GAPDH) blot serves as a loading control. Graphs are results of densitometric analyses of Aβ (bottom left) or APP holoprotein (bottom right) signals normalized by the glyceraldehyde 3-phosphate dehydrogenase signals (**P < 0.01, #P = 0.72, n = 8 per genotype, two-tailed Student’s t-test). (E) Immunoprecipitation (IP) of brain homogenates with 6E10 followed by western blotting (WB) with 22C11. APP Holo = APP holoprotein and holoprotein derivatives; CRND8 = TgCRND8; CstB = cystatin B. Scale bars: 500 µm (A1, A2, B1 and B2).