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. 2010 Oct 27;285(53):41232–41243. doi: 10.1074/jbc.M110.156273

FIGURE 7.

FIGURE 7.

Hydrolytic activity toward cell wall and peptidoglycan preparations. A and B, hydrolytic activity toward B. subtilis cell wall (A) and peptidoglycan (B). C, hydrolytic activity toward S. aureus cell wall and peptidoglycan preparations. D, hydrolytic activity toward S. thermophilus cell wall and peptidoglycan. The hydrolytic activity of CwlP-SLTM23 toward S. aureus and S. thermophilus cell walls is shown in C and D, respectively. Open circles, no enzyme (A) or CwlP-SLTM23 cell wall directed activity (C and D); closed squares, CwlP-SLT activity toward cell walls (A) and peptidoglycans (B–D); closed triangles, CwlP-M23 activity toward cell walls (A) and peptidoglycans (B–D); closed circles, CwlP-SLTM23 activity toward cell walls (A) and peptidoglycans (B–D); closed diamonds, mixture of CwlP-SLT and CwlP-M23 activities toward cell walls (A) and peptidoglycans (B–D). CwlP-SLT, CwlP-M23, and mixtures of CwlP-SLT and CwlP-M23 exhibited no activity toward S. aureus and S. thermophilus cell walls, in common with CwlP-SLTM23 (open circles in C and D). The error bars indicate the standard deviations from three independent experiments.