FIGURE 2.
A novel, non-consensus polo-box binding sequence in Dbf4. A, Dbf4 N-terminal deletion mutants were constructed to determine the minimal region (residues 78–96) required for a two-hybrid interaction with the PBD. B, a biotinylated Dbf4 peptide (residues 73–96, panel C) was tested for interaction with purified His6-PBD using the AlphaScreen assay. Data represent the average of three independent experiments ± S.D. C, the Dbf4(73–96)-PBD interaction was competed with the indicated non-biotinylated, wild type Dbf4 peptides (P1–P4), or mutant Dbf4 peptides as indicated in (D and E).