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. 2010 Oct 29;285(53):41244–41254. doi: 10.1074/jbc.M110.155242

FIGURE 5.

FIGURE 5.

The Cdc5 pincer residues are not required for yeast viability. A, the cdc5-HK and cdc5-WHK mutants complemented a cdc5Δ by plasmid shuffle into M1672 (cdc5Δ::kanMX6/pMW536[CDC5 URA3]) evidenced by growth on 5-fluoro-orotic acid plates and at various temperatures on YPD plates after loss of pMW536. B, growth curves of M1672 strains containing only the indicated CDC5 alleles in YPD at 30 °C are shown. C, the M1672-transformed strains in panel A were spotted onto YPD plates with/without benomyl (top). WT (M138), cdc5-HK (M3502), cdc5-5 (M1680), and cdc15-4 (M1999) strains containing integrated alleles were similarly spotted onto YPD with/without benomyl (bottom). D, representative tetrads from diploid strains of genotype cdc5-HK/CDC5 dbf2–1/DBF2, cdc5-HK/CDC5 cdc15-4/CDC15 TAB6-1/CDC14, or cdc5-HK/CDC5 cdc14-1/CDC14 that were sporulated and dissected onto YPD plates at 25 °C. Recombinant genotypes are indicated.