FIGURE 5.

Halothane activates Kir3.2(F192M) and Kir3.2(L273I) in a dose-responsive manner. A, sample TEVC recording from an oocyte expressing Kir3.1/Kir3.2(F192M) and its response to varying concentrations of halothane. The oocyte was held at −80 mV, and currents were measured in high potassium (HK). Varying concentrations of halothane were applied, and barium was used at the end to determine basal currents. The dose-response curve for halothane activation of this channel is seen on the right. The EC₅₀ for activation was 0.151 mm. B, sample TEVC recording from an oocyte expressing Kir3.1/Kir3.2(L273I) and its response to varying concentrations of halothane. The EC₅₀ for activation was 0.862 mm.