FIGURE 2.

CPAF mediates p65 cleavage. A, p65 is cleaved in cells expressing active CPAF. T-REx-293 CPAF K6 cells (CPAF K6) were induced to express active CPAF with 5 ng/ml AHT and 1 μm CM for the times indicated. Lysates were subjected to Western blotting. Shown is a representative result of three independent experiments. F, CPAF-specific cleavage products; *, unspecific background bands. CPAFc, C-terminal autoprocessing product of CPAF. B, Inhibition of CPAF activity blocks p65 cleavage. T-REx-293 CPAF K6 cells were pretreated with the proteasomal inhibitors lactacystin (25 μm) or epoxomicin (2.5 μm) for 30 min. before CPAF was induced with AHT and CM. After eight h of induction cells were harvested and subjected to Western blotting. Shown is a representative result of six independent experiments.