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. 2010 Oct 28;285(53):41348–41355. doi: 10.1074/jbc.M110.188110

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — Production of recombinant forms of PpgS and Rv3632 in M. smegmatis mc²155. The production of a recombinant C-terminal His₆-tagged form of PpgS from M. tuberculosis was detected by Coomassie Blue staining (A) and Western blot using a monoclonal anti-His tag antibody (B) in the mc²155/pVVppgS strain (lanes 3) but not in the control strain, mc²155/pVV16 (lanes 1). The production of a recombinant C-terminal His₆-tagged form of Rv3632 from M. tuberculosis was detected by Western blotting (B) in the mc²155/pVVRv3632 strain (lanes 2) and mc²155/pVVppgS-Rv3632 strain (lanes 4) but not in the control strain, mc²155/pVV16 (lanes 1).