FIGURE 1.

Glucagon-dependent induction of Socs3 in primary hepatocytes. A, primary hepatocytes were treated with IL-6 (20 ng/ml) or 8-br-cAMP (0.1 mm) for 2 h. Relative Socs3 expression was measured by qRT-PCR. Time-dependent (B) and concentration-dependent (C) expression of Socs3, Pck1, and G6pc in response to glucagon (10 nm) were measured. D, Socs3 and Pck1 mRNA levels were measured in liver from mice fasted 24 h, or refed for 4 h following a 24 h fast. Data are normalized to fed mice. Data represent the mean ± S.D., n = 3–6. **, p < 0.01.