FIGURE 6.

Resveratrol alters SIRT1 and HIF-1α expression in liver tissue when administered prior to the endotoxin challenge. Mice were treated as described in the legend to Fig. 5. Blood (A) and liver tissue (B–F) were obtained at 4 h post-infusion. SIRT1 transcripts levels in peripheral blood leukocytes (A) and liver tissue (B) were examined by quantitative PCR. Data are expressed as means ± S.E. (n = 4) relative to the untreated group. C–E, liver tissue samples normalized for protein content were used for Western blot analysis with the indicated antibodies. Statistical significance was determined by one-way ANOVA. Veh, vehicle; Res, resveratrol.