FIGURE 2.
Voltage dependence of TRPV1 mutants. A, representative whole cell patch clamp current traces in response to a family of voltage steps in HEK293T cells transfected with wild-type and TRPV1 mutants in the absence (open symbols) or presence of 10 μm capsaicin (filled symbols). B, representative Boltzmann fits to conductances shown in A, obtained from steady-state currents at the end of the pulse (indicated by symbols in A), normalized to maximal conductance Gmax in 10 μm capsaicin. C, V1/2 for wild-type TRPV1 and indicated mutants in the absence (open bars) or presence of 10 μm capsaicin (filled bars). The asterisks indicate significance level. Under control conditions, some mutants did not reach half-maximal activation at voltages up to 200 mV. D, summary of voltage-independent component of capsaicin-induced gating (upward bars) and Gmax in 10 μm capsaicin (downward bars). Each bar is the mean ± S.E. (error bars); n > 4.