FIGURE 2.

Lysenin binding reveals decreased levels of plasma membrane SM in ldlC cells. A, WT and ldlC CHO cells were fixed, treated with lysenin at room temperature for 1 h, and then treated with anti-lysenin antiserum followed by the fluorescent secondary antibody. Cells were observed in a confocal microscope. B, the number of lysenin aggregates per cell was determined by counting 30 cells in each condition using ImageJ software, as indicated under “Experimental Procedures.” Scale bar, 10 μm.