FIGURE 1.

Time course and concentration dependence of sPLA₂-X mediated eicosanoid synthesis by eosinophils. A, following treatment with 100 nm exogenous sPLA₂-X, there was an increase in [³H]AA release by eosinophils over time that reached a plateau 15 min after addition of the enzyme (p < 0.0001). The [³H]AA release is expressed in terms of percentage of total [³H]AA incorporated. B, eosinophil synthesis of CysLTs increased over time after treatment with 100 nm exogenous sPLA₂-X, reaching a maximum 20 min after the addition of the enzyme (p = 0.002). C, the levels of CysLTs following treatment of eosinophils for 20 min with buffer control or 1, 10, or 100 nm sPLA₂-X increased with increasing concentrations of the recombinant enzyme (p = 0.008). Error bars, S.E.