FIGURE 3.
Sedimentation velocity experiments of A. aeolicus purified supercomplex. A, superimposition, for the supercomplex at 1.75 mg/ml in buffer A, of selected experimental sedimentation profiles obtained at 413 nm during 6 h at 42,000 rpm, at 12 °C, in 3-mm optical path length cell and of their modeled profiles with the c(s) analysis. B, corresponding residuals. C, superposition of the c(s) analysis for the supercomplex at 1.75, 0.35, and 0.10 mg/ml followed at 413 nm. D, result of the c(s) analysis for the supercomplex at 0.35 mg/ml followed at 278 and 413 nm and using interference optics. s20,w values were calculated using v̄ = 0.75 ml/g, corresponding to the protein part. The errors in s20,w related to the choice of s20,w are less than 3%.