. 2010 Oct 22;285(53):41815–41826. doi: 10.1074/jbc.M110.167841

TABLE 3.

Comparison of the properties of the isolated Sqr and the Sqr integrated in the supercomplex

State of the Sqr	Isolated (Sqr_free, this study)	Integrated in the supercomplex (this study)	Isolated (from Ref. 46)
Oligomeric state on blue native gel	4 states at 58, 106, 175 and 240 kDa	2 states at 58 and 106 kDa	4 states at 50, 90, 150, 280 kDa
t½ at 80 °C	18 h	15 h	32 h
k_cat (s⁻¹)^a	75^b	60^b	59^c
K_m (DB)	1.66 ± 0.68 μm^b	0.71 ± 0.4 μm^b	2.16 ± 0.19 μm^d
k_cat/K_m^a	45.2 s⁻¹·μm⁻¹^b	84.5 s⁻¹·μm⁻¹^b

^a Kinetic parameters were calculated using the monomeric concentration of isolated Sqr and on the basis of one monomeric Sqr in the supercomplex.

^b Data were determined at 25 °C.

^c Data were determined at 40 °C.

^d Temperature was not specified.