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TPSF inhibits growth of MCF-7 cells in soft agar. 5000 MCF-7 cells were plated into top agar containing 1 pm E2 (left) or E2 + 10 μm TPSF (right) as described under “Experimental Procedures.” After 16 days colonies were photographed at 5× magnification and counted. Photographs are representative of the entire plate and of duplicate experiments.
