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Megalin binds AGT. A, silver staining and Western blot of megalin pulldown assay using GST, GST-RAP, and GST-AGT, showing that megalin binds to RAP and AGT. B, pulldown assay using Myc-tagged LBR 1–4 and the Myc-tagged C terminus (C-term) of megalin. Protein fragments of Myc-tagged LBR and C terminus (left, B). As opposed to the C terminus, all four LBR bind to GST-RAP (middle, B) and GST-AGT (right, B).
