FIGURE 5.

Intracellular localization of AGT in the proximal tubule. A–D, double immunofluorescence staining of AGT (green) with anti-clathrin antibody staining clathrin-coated pits or vesicles (red, A), anti-EEA1 antibody staining early endosomes (red, B), anti-Rab11 antibody staining recycling endosomes (red, C), and anti-LAMP-2 antibody staining lysosomes (red, D). In the merge pictures, sites of double staining are indicated by arrowheads. The dotted lines mark the position of the apical plasma membrane beneath the brush-border membrane and the basal lamina, respectively, as indicated. Bar, 5 μm.