FIGURE 6.

Proximal tubular processing of AGT and Ang II. Opossum kidney cells cultured in dual chamber devices were incubated apically with 10 μg of AGT or 10 μg of Ang II for 1 h. Samples from the lower chambers were analyzed. A, in comparison with control, the amount of transcytosed AGT is significantly reduced upon RAP or colchicine administration (Western blot evaluation). B, transcytosed Ang II is significantly lower upon RAP, valsartan, or colchicine administration (competitive ELISA). The values are the means ± S.E. (n = 6). *, p < 0.05.