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. 2010 Oct 21;285(53):41935–41946. doi: 10.1074/jbc.M110.150284

FIGURE 8.

FIGURE 8.

ACE and ACE2 expression in megalin conditional knock-out mice. A, double immunofluorescence staining of ACE in Cre(−) kidney. The lower dotted lines indicate the border between cortical labyrinth and medullary rays/outer stripe; signal is high in the latter. B, in PST of Cre(+) kidney, megalin-deficient cells exhibit reduced ACE signal in BBM compared with megalin-expressing cells. C, BBM fractions show an overall mild reduction of ACE in Cre(+) compared with Cre(−) by Western blot. D, double immunofluorescence staining of ACE2 in Cre(−) kidney. The lower dotted lines indicate transition from outer stripe to inner stripe of outer medulla. E, in Cre(+) kidney, megalin-deficient cells exhibit increased ACE2 signal in BBM compared with megalin-positive cells. F, BBM fractions show higher ACE2 abundance in Cre(+) compared with Cre(−) by Western blot. The upper dotted lines mark the renal capsule. The values are the means ± S.E. (n = 5). *, p < 0.05. Scale bar in A and D, 20 μm; scale bar in B and E, 10 μm.

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