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. 2010 Oct 14;285(53):41961–41971. doi: 10.1074/jbc.M110.169607

FIGURE 2.

FIGURE 2.

LIN28-regulatory function of mir-9 and mir-30 was validated by the 3′-UTR reporter assay. A, schematic diagram of the mir-125, let-7, mir-30, and mir-9 binding sites in the LIN28 3′-UTR. The seeding sequences (marked in gray) were broadly conserved among different species. Hsa, Human; Ptr, chimpanzee; Mml, Rhesus; Mmu, mouse; Rno, Rat; Cpo, Pig; Ocu, rabbit; Eeu, Hedgehog; Cfa, Dog; Eca, Horse; Bta, Cow; Dno, armadillo; Laf, elephant; Mdo, opossum. B and C, summary of the reporter assays on the wild type and mir-9 binding site mutant reporter (B) and mir-30 binding site mutant reporter (C) in A2780 (LIN28-positive) and HeLa (LIN28-negative) cells. WT, wild type LIN28 3′-UTR reporter; mut9, mir-9 binding site mutant LIN28 3′-UTR reporter; mut30, mir-30 binding site mutant LIN28 3′-UTR reporter. Overexpression of mir-9 or mir-30 was able to significantly (*, p < 0.05) reduce luciferase activity in the wild type but not the binding site mutant LIN28 3′-UTR reporters.