X-ray patterns for the various genotypes during perfusion of the nerves with physiological saline, recorded immediately after dissection, and after 5 and 20 h. A, WT; B, S63C(+/+); C, S63C(+/−); D, S63C(−/−); E, P0(+/−); and F, P0(−/−). Each spectrum is the data collected for a duration of 10 min, and Bragg orders 2–5 are indicated if visible. A, WT showed a stable period of 175 Å throughout the 20-h perfusion. B, S63C(+/+) showed a stable period similar to that of the WT. C, S63C(+/−) showed the formation of a new reflection (*) as the perfusion time increased. D, S63C(−/−) showed a constant swollen period of ∼207 Å for the entire duration. E, P0(+/−) showed a stable period of 178 Å for at least 5 h but then developed a swollen phase. F, P0(−/−) showed a constant period of 191 Å, which was smaller than that observed for S63C(−/−) (207 Å).