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. 2010 Oct 11;285(53):42001–42012. doi: 10.1074/jbc.M110.166967

TABLE 2.

Stability of sciatic nerve myelin during perfusion

Myelin structure in nerves mounted in a perfusion chamber was assessed by XRD; patterns were recorded immediately after dissection and at 5 and 20 h during the perfusion with physiological saline. See legend to Table 1 for details.

Time Dimensions
Δ/d Myelin M/(M+B)
d cyt lpg ext
Å %
WT
    10 min 174.7 31 48 47 0.023 0.38
    5 h 174.7 31 48 47 0.023 0.42
    20 h 174.7 31 48 47 0.023 0.41

S63C(+/+)
    10 min 175.3 31 49 46 0.023 0.30
    5 h 175.1 31 49 46 0.023 0.31
    20 h 174.4 30 49 46 0.023 0.29

S63C(+/−)
    10 min 175.9 33 47 49 0.028 0.23
    5 h 175.9 33 47 49 0.023 95 0.27
196.5 5
    20 h 174.7 31 47 49 0.029 88 0.25
220.5 12

S63C(−/−)
    10 min 206.0 0.049 0.14
    5 h 208.4 0.048 0.15
    20 h 205.4 0.054 0.21

P0(+/−)
    10 min 179.5 33 48 50 0.028 0.29
    5 h 180.6 33 50 48 0.028 0.27
    20 h 181.9 34 49 50 0.033 79 0.21
204.6 21

P0(−/−)a
    20 min 191.2 0.037 0.10
    5 h 191.7 0.042 0.09

a P0(−/−) sciatic nerve was too weak to withstand lengthy perfusion, so only data at 5 h are shown.