TABLE 3.

WT and P0 S34C sciatic nerves incubated with TCEP

The myelin structure was assessed by XRD in sciatic nerves incubated at physiological ionic strength (i.e. 154 mm NaCl, 5 mm Tris buffer, pH 7.4) with different concentrations of TCEP (see “Experimental Procedures”).

TCEP	Dimension				Δ/d	Myelin	M/(M+B)
	d	cyt	lpg	ext
mm	Å					%
WT
0	175.0	31	49	46	0.017		0.30
10	175.0	31	49	46	0.023		0.34
50	177.0	34	49	45	0.023		0.38
S63C(+/−)
0	176.0	33	47	49	0.023	80	0.30
	218.7					20
10	177.0	33	49	46	0.023		0.27
50	176.0	33	48	47	0.023		0.27
S63C(−/−)
0	211.0	33	46	86	0.033		0.19
10	187.3					26	0.16
	222.3					74
50	180.0	32	49	50	0.028		0.12