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. 2010 Oct 19;285(53):42033–42045. doi: 10.1074/jbc.M110.149831

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Hsp70 is required for the interaction between hTERT and CHIP. A, H1299 cells transfected with hTERT-HA and scrambled or Hsp70 siRNA (siHsp70-1 or siHsp70-2) were treated with 10 μm MG132 for 2 h. Lysates (input) and anti-HA immunoprecipitates (IP) were analyzed by immunoblotting as indicated. B, H1299 cells transfected with scrambled or Hsp70 siRNA were analyzed for telomerase activity by the TRAP assay. C, H1299 cells transfected with scrambled or Hsp70 siRNA were treated with 10 μm MG132 for 2 h and subjected to indirect immunofluorescence with anti-hTERT (green) antibody, followed by fluorescent microscopic observation. The nuclei were stained with 4,6-diamino-2-phenylindole (DAPI, blue). ITAS represents the internal telomerase assay standard.