FIGURE 1.

Decorin bioactivity in MDCK cells. A, immunoblot of MDCK cells treated with or without decorin (100 nm) for 6 h. Cell lysates were separated by 8% SDS-PAGE and immunoblotted for Met and β-catenin with GAPDH as loading control. Proteins were visualized with IR-Dye-labeled secondary antibodies and quantified using Odyssey Infrared Imaging system (Li-Cor). B–D, immunofluorescence images of MDCK cell untreated (control) or treated with either HGF (50 ng/ml, ∼0.71 nm) or decorin (100 nm) for 6 h. E–H, decorin attenuates HGF/SF induced MDCK cell scattering after 14-h incubation with (F and H) or without (E and G) 500 nm decorin in the presence (G and H) or absence (E and F) of HGF (0.71 nm). I, quantification of scattered cells after 14 h of HGF and decorin treatment. The values represent the mean ± S.E. (n = 40 each, ***, p < 0.001).