FIGURE 3.

Decorin inhibits migration via the Met receptor and evokes caveolin-mediated internalization of Met. A, motility assay perfomed with a monolayer of HeLa cells scratched to cause a wound. Cells were treated for 18 h with 500 nm decorin or 0.71 nm HGF. Where indicated, 10 μg/ml H9786, a Met-blocking antibody, was added 30 min before treatment. Representative photos are shown. Bar, 60 μm. B, confocal images showing the Met receptor (green) and caveolin (red) in HeLa cells treated with or without 500 nm decorin or 0.71 nm HGF for 5 h. C, HeLa cells under the same treatment as shown in B, but stained for Met (green) and clathrin (red). All images were taken with the same exposure and gain. White arrows indicate co-localization. Bar, 10 μm.