FIGURE 7.

Systemic delivery of decorin protein core inhibits Met expression in two tumor xenografts. A, bar graph showing inhibition of A431 squamous tumor growth in SCID mice at day 23 of decorin treatment (5 mg/kg). Data represent the mean ± S.E. (n = 8, **, p < 0.01). B, representative immunoblot of A431 tumor lysates showing down-regulation of Met expression following decorin treatment. Tubulin was used as loading control. C and D, same as in A and B using MTLn3 mammary carcinoma xenografts. E-I, detection of Met (green) using immunofluorescence of frozen sections from A431 tumor xenografts of vehicle (control) or decorin-treated mice. All the micrographs were taken using the same exposure and gain. Mice bearing A431 tumor xenografts were treated with intraperitoneal injections of decorin (5 mg/kg) every other day for 26 days. Bar, 250 μm. G and J, three-dimensional surface plots generated with ImageJ software depicting levels of Met expression corresponding to the staining above. The scale bars for signal intensity are included in the top left corner.