FIGURE 2.

Alignment of the amino acid sequences of the SH2 domains of GRB7, GRB10, and GRB14. The α-helices and β-strands of GRB10 SH2 are highlighted with red lines and blue arrows, respectively. The underlined peptide of GRB10 is the F β-strand, which forms an antiparallel β-sheet with the C β-strand of NEDD4 C2 in the NEDD4 C2-GRB10 SH2 complex. The residues buried in the interface with NEDD4 C2 are shown with red letters. The two residues in interface I (Lys⁵⁰⁵ and Asn⁵¹⁹) chosen for mutation are highlighted as red boldface letters.