FIGURE 2.
The C terminus of Alb3 binds to the chromodomains 2 and 3 of cpSRP43. CD2CD3, A3CT, cpSRP43Δ2Δ3, and mixtures of CD2CD3 and A3CT (molar ratio 1:2) or cpSRP43Δ2Δ3 and A3CT (molar ratio 1:2) were subjected to analytical size exclusion chromatography in 20 mm Hepes/NaOH pH 7.5, 200 mm NaCl, 2 mm MgCl2, 1 mm EDTA, 1 mm DTT using a Superdex200 (10/300) column (shown on the left). Protein-containing fractions were analyzed by SDS-PAGE and are shown on the right. In the presence of A3CT, CD2CD3 shifts to smaller elution volume indicating complex formation between A3CT and CD2CD3.