Figure 4.

Effect of TRG on PI3K/Akt Pathway in the presence or absence of serum. (A) Huh-7 cells were treated with 25 μM TRG in the absence (-) or presence (+) of serum for the indicated periods of time. Equal amounts of cell extracts were analyzed by Western Blot analysis utilizing antibodies against pAkt^Ser473, Akt or GAPDH. (B) Western Blot analysis of the cell extracts treated with TRG in serum-deficient media following normalization of total AKT levels. (C) Cells treated with increasing concentrations of TRG for 6 hours in serum deficient media were subjected to Western Blot analysis as in A.