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. 2010 Dec 13;5:20. doi: 10.1186/1750-2187-5-20

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Copyright ©2010 Mishra et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<url>http://creativecommons.org/licenses/by/2.0</url>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effect of PI3K and Akt inhibition on TRG-induced apoptosis resistance in Hep3B cells. (A) Subconfluent Hep3B cells were treated with 25 μM TRG in the presence (+) or absence (-) of serum for the indicated periods of time. Western Blot analyses were performed with the antibodies indicated. (B) Western analysis of Hep3B extracts treated with TRG in serum-containing media for 24 hours following a 1 hour pretreatment with none (lane 2), LY294002 (lane 3), LY303511 (lane 4), or Akt inhibitor VIII (lane 5).