Figure 2.

Pattern of lanes obtained by computer digitization of the gel band densities. Histograms correspond to lanes in Fig. 1. The upper plot represents the profile of intensity of cleavage (I) for lane 6 and is followed by the same type of profiles built for lanes 11 and 17. The last two plots represent profiles of the relative intensity of cleavage (R) for lanes 17 and 6, obtained by the moving-average method, to demonstrate the normalization procedure described in Materials and Methods.