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. 2011 Jan;137(1):59–79. doi: 10.1085/jgp.201010532

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© 2010 Srinivasan et al.

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Figure 3. — Effect of the β2 M3–M4 loop ER retention/retrieval motif on PM trafficking of α4β2 nAChRs. (A) Representative TIRFM images of α4eGFP plus β2-365AAQA368–transfected cells. Nicotine treatment conditions are indicated for each image; bars, 10 µM. (B) Integrated density for α4eGFP plus β2wt– and α4eGFP plus β2-365AAQA368–transfected cells. Transfected β2 subunits and nicotine treatment conditions are indicated below the x axis; the numbers of imaged cells are indicated within the parentheses; error bars show 99% confidence intervals. (C, 1 and 2) Line graphs plotting the pixel number versus fluorescence intensity for whole TIRF footprint (ER + PM) or the ER for α4eGFP plus β2-365AAQA368–transfected cells with and without nicotine as indicated. (D) Integrated density ratios of whole TIRF footprint to ER for α4eGFP β2wt and α4eGFP β2-365AAQA368 nAChRs; error bars are SEM. The transfected β2 subunit and nicotine treatment conditions are indicated below the x axis. The numbers of imaged cells are indicated in parentheses.