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. 2010 Dec 27;191(7):1251–1260. doi: 10.1083/jcb.201005065

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© 2010 Breuer et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 2. — Hurp^−/− oocytes are delayed in bipolarization and undergo spindle collapse. (A) Hurp^−/− oocytes present spindle defects. For A, B, and D, oocytes express EB3-GFP (green) and H2B-RFP (red). Asterisks, polar bodies. (B) MTs are not evenly distributed around chromosomes after NEBD in Hurp^−/−. Mean projections of 10 Z planes are shown. (C) Setup of spindle bipolarity is delayed in Hurp^−/− oocytes. Bipolarity was scored when two poles were distinguishable. It is delayed in Hurp^−/− oocytes, starting at 297 ± 101 min instead of 209 ± 74 min (**, P = 0.0334). Lines indicate the mean time for bipolarity setup. (D) Transient spindle collapse in Hurp^−/− oocytes during MI. Bipolarity reappears at 14 h and 40 min after NEBD. (E) Unstable spindle length in Hurp^−/− oocytes. Length was determined by longitudinal line scans of images from wt (n = 12) and Hurp^−/− (n = 14) oocytes. Representative curves are shown. All times are given in hours and minutes after NEBD. Bars, 10 µm.